Type C or D of ASEBIR.Įmbryos of deficient quality according to morphokinetic criteria established (28) for EmbryoScope Time-Lapse if this incubator has been used. Embryo D5 or blastocyst: Inner cell mass absent, with few cells and difficult differentiation, Trophectoderm with very few cells. Embryo D2 and D3: classified as Type C or Type D according to ASEBIR criteria. Low or abnormal Fertilization Rate despite semen count > 3 million/mL.ĭeficient quality embryos according to morphological criteria established by ASEBIR:.BMI 70% of the embryos obtained being included in the worst prognosis category according to any of the following criteria:.Previous IVF cycle with 5 or more metaphase II oocytes after retrieval.The patients must have read, understood and signed the ICF.Other: Autologous mitochondria with ICSI Other: STANDARD ICSI PROCEDURE Following an interim analysis of outcomes, an additional 130 patients may be added, for a total of 190 patients. Using an adaptive design, retrieved oocytes of approximately 60 patients will be randomized in the first part of the study to two treatment groups standard ICSI procedure without mitochondrial supplementation and ICSI with autologous mitochondrial supplementation. This improvement in embryo quality will be determined through on-going pregnancy rate after treatment and/or improvement in embryo quality according to morphological (ASEBIR-'Association for the study of Biology in Reproductive Science), morphokinetic criteria and in Preimplantation Genetic Screening. The principle objective is to improve embryo quality through autologous micro-injection of mitochondria isolated from Ovarian stem cells into the oocytes themselves, as a complementary ICSI technique in patients with low embryo quality in previous IVF cycles and in those who did not bear children. Why Should I Register and Submit Results?.

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